2EHT

Crystal structure of acyl carrier protein from Aquifex aeolicus (form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-10-24
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	30.312, 37.042, 61.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.196
Rwork	0.196
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l0h
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.050	0.117
Number of reflections	14150
<I/σ(I)>	29.7	14.11
Completeness [%]	99.6	97.1
Redundancy	15.5	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	295	50mM zinc acetate, 20%(w/v) PEG 3350, pH 6.4, microbatch, temperature 295K
1	MICROBATCH	6.4	295	50mM zinc acetate, 20%(w/v) PEG 3350, pH 6.4, microbatch, temperature 295K
1	MICROBATCH	6.4	295	50mM zinc acetate, 20%(w/v) PEG 3350, pH 6.4, microbatch, temperature 295K