2EHP
Crystal Structure of a Putative protein (AQ1627) from Aquifex aeolicus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-11-28 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97884, 0.9000, 0.97973 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.353, 53.873, 60.168 |
| Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.300 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.034 | 0.204 |
| Number of reflections | 51115 | |
| Completeness [%] | 89.3 | 85.7 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 40% Ethanol, 5% PEG1000, Phosphate-Citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
