2EH9
Crystal structure of the HBAF250B at-rich interaction domain (ARID)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-27 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 42.887, 42.887, 144.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.890 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cxy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.361 |
| Number of reflections | 9616 | |
| <I/σ(I)> | 36.3 | 3.7 |
| Completeness [%] | 97.5 | 81.7 |
| Redundancy | 13.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | DNA(dCGCGAATTCGCG), 120MM MGCL2, 60MM TRIS-HCL, 18% PEG4000, 20MM ZNCL2, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
