2EGV

Crystal structure of rRNA methyltransferase with SAM ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	298
Detector technology	CCD
Collection date	2006-10-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	62.364, 76.976, 97.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.010 - 1.450
R-factor	0.218
Rwork	0.218
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	79269
<I/σ(I)>	21.4595	2.68627
Completeness [%]	94.4	89.3
Redundancy	4.90016	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M magnesium acetate tetrahydrate 0.1M sodium cacodylate pH6.5 20% polyethylene glycol 8000 , VAPOR DIFFUSION, SITTING DROP, temperature 293K