2EGJ
Crystal Structure of Hypothetical Protein(AQ1494) from Aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-12-16 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97943 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.468, 69.426, 84.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.110 - 1.800 |
R-factor | 0.19838 |
Rwork | 0.197 |
R-free | 0.22062 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2egi |
RMSD bond length | 0.013 |
RMSD bond angle | 1.315 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.056 | 0.279 |
Number of reflections | 29686 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.9 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.2M Sodium chloride, 0.1M Phosphate-citrate, 50% PEG200, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |