2EG3
Crystal Structure of Probable Thiosulfate Sulfurtransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-02 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.376, 72.464, 116.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.130 - 1.800 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.22300 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.072 | 0.381 |
Number of reflections | 51337 | |
<I/σ(I)> | 24.8 | 6.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6M AMMONIUM SULFATE, 0.1M HEPES BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |