2EG1
The crystal structure of PII protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-29 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 81.040, 81.040, 81.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.520 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gw8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.039 | 0.232 |
| Number of reflections | 8368 | |
| <I/σ(I)> | 81.25 | 14.91 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.2 | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 291 | Citrate, Ammonium sulfate , pH5.3, microbatch method , temperature 291K |
