2EFO
Crystal structure of Tyr77 to Ala of ST1022 from Sulfolobus tokodaii 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.902, 103.902, 74.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.710 - 2.400 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.092 | 0.488 |
| Number of reflections | 10745 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.6 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20% PolypropyleneGlycol, 30% Isopropanol, 0.07M Sodium citrate, 200mM ndSb-221, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
