2EBN

CRYSTAL STRUCTURE OF ENDO-BETA-N-ACETYLGLUCOSAMINIDASE F1, AN ALPHA(SLASH)BETA-BARREL ENZYME ADAPTED FOR A COMPLEX SUBSTRATE

Experimental procedure

Spacegroup name	P 61
Unit cell lengths	70.640, 70.640, 103.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.158
Rwork	0.158
RMSD bond length	0.016
RMSD bond angle	0.033 *
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.030 *
Total number of observations	141175 *
Number of reflections	19158 *
Completeness [%]	93.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	cacodylate	100 (mM)
3	1	reservoir	PEG8000	14-18 (%(w/v))
4	1	reservoir	zinc acetate	100-200 (mM)
5	1	reservoir	cacodylate	100 (mM)