2EAQ
Crystal structure of PDZ domain of KIAA0858 (LIM), MS0793 from Homo sapiens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794, 0.9796, 0.9644 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.996, 61.311, 27.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.930 - 1.460 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.19400 |
| Structure solution method | MAD |
| RMSD bond length | 0.039 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 13707 | |
| <I/σ(I)> | 30.94 | 8.28 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 13.2 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 12% (v/v) PEG3350, HEPES-Na (pH7.5), 10mM NiSO4., VAPOR DIFFUSION, SITTING DROP, temperature 293K |
