2EA1

Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	1997-06-12
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	43.200, 49.870, 54.520
Unit cell angles	90.00, 96.37, 90.00

Refinement procedure

Resolution	54.230 - 1.800
R-factor	0.22
Rwork	0.218
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.932
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.230	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.077	0.182
Number of reflections	18428
Completeness [%]	85.7	36.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	298	25% PEG 3350, 0.1M MES, 0.1M AMMONIUM ACETATE, 1MM MAGNESIUM CHLORIDE, 10% GLYCEROL, 4MM EDTA, VAPOR DIFFUSION, HANGING DROP, pH 6.10, temperature 298.0K