2E85
Crystal Structure of the Hydrogenase 3 Maturation protease
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97921, 0.97955, 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.425, 54.984, 67.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.084 | 0.231 |
| Number of reflections | 36372 | |
| <I/σ(I)> | 19.54 | 5.84 |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M CaCl2, 0.1M Sodium Hepes, 28% PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
