2E7T
Crystal structure of basic winged bean lectin in complex with a blood group trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.548 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 157.729, 91.130, 73.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.650 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.24400 |
| Starting model (for MR) | 1wbl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.126 | 0.460 |
| Number of reflections | 30585 | |
| <I/σ(I)> | 14.5 | 3.7 |
| Completeness [%] | 96.9 | 97.4 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.05 | 293 | 6-7%(w/v) PEG 3350, 10%(v/v) isopropenol, 20mM PBS, pH 7.05, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
