2E7T
Crystal structure of basic winged bean lectin in complex with a blood group trisaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.548 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 157.729, 91.130, 73.718 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.000 - 2.650 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.24400 |
Starting model (for MR) | 1wbl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.126 | 0.460 |
Number of reflections | 30585 | |
<I/σ(I)> | 14.5 | 3.7 |
Completeness [%] | 96.9 | 97.4 |
Redundancy | 4.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.05 | 293 | 6-7%(w/v) PEG 3350, 10%(v/v) isopropenol, 20mM PBS, pH 7.05, VAPOR DIFFUSION, HANGING DROP, temperature 293K |