2E7L
Structure of a high-affinity mutant of the 2C TCR in complex with Ld/QL9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 113.540, 113.540, 177.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.2251 |
| Rwork | 0.224 |
| R-free | 0.24562 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oi9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.780 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.490 |
| Rmerge | 0.117 | 0.665 |
| Number of reflections | 39001 | |
| <I/σ(I)> | 29 | 4.4 |
| Completeness [%] | 99.9 | 99.06 |
| Redundancy | 12 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M ammonium phosphate, 20% PEG 3350, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
