2E77
Crystal structure of L-lactate oxidase with pyruvate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.841, 118.566, 107.237 |
| Unit cell angles | 90.00, 121.07, 90.00 |
Refinement procedure
| Resolution | 37.400 - 1.900 |
| R-factor | 0.17903 |
| Rwork | 0.176 |
| R-free | 0.22682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2du2 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.661 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.053 |
| Number of reflections | 113367 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
