2E5F
Crystal Structure of the PH0510 protein from Pyrococcus horikoshii OT3 in complex with phosphate ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.80 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.009, 79.366, 67.440 |
| Unit cell angles | 90.00, 105.31, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.350 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dec |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.076 | 0.276 |
| Number of reflections | 135393 | |
| <I/σ(I)> | 10.6 | 3.12 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 5.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.9 | 295 | 20%(w/v) PEG 3350, 0.2M diammonium hydrogen phosphate, pH 7.9, microbatch, temperature 295K |
