2E5A
Crystal Structure of Bovine Lipoyltransferase in Complex with Lipoyl-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.439, 81.439, 120.532 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.22092 |
| Rwork | 0.219 |
| R-free | 0.26270 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.051 | 0.470 |
| Number of reflections | 27589 | |
| <I/σ(I)> | 58.2 | 5.2 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 8.6 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
