2E4Z
Crystal structure of the ligand-binding region of the group III metabotropic glutamate receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.431, 92.431, 114.332 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 3.300 |
| R-factor | 0.27369 |
| Rwork | 0.270 |
| R-free | 0.32395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ewk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.963 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.112 | 0.540 |
| Number of reflections | 8887 | |
| <I/σ(I)> | 5.4 | 1.4 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 0.02M Citrate, 0.08M MES, 0.01M Glutamate, 2.2M ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
