2E3W

X-ray structure of native RNase A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2006-08-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.90020
Spacegroup name	P 32 2 1
Unit cell lengths	64.167, 64.167, 63.626
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.050
R-factor	0.19329
Rwork	0.193
R-free	0.20783
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1izp
RMSD bond length	0.014
RMSD bond angle	1.570
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.050
Number of reflections	69871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.89	292	Well solution: Combination of Hampton Index Reagents 3 and 9, Final solution: 0.1 M Bis-tris, 1.0 M ammonium sulfate, 1.5 M NaCl, pH 5.89, Drop: 2 microliter well solution + 2 microliter 10 mg/ml protein solution in water, VAPOR DIFFUSION, HANGING DROP, temperature 292K