2E3R
Crystal structure of CERT START domain in complex with C18-ceramide (P1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.459, 50.134, 62.652 |
| Unit cell angles | 77.54, 70.96, 81.85 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.220 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 59501 | |
| Completeness [%] | 95.5 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP |
