2E3C
Crystal structure of the catalytic domain of pyrrolysyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 64 |
Unit cell lengths | 104.129, 104.129, 71.234 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.100 - 2.650 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2DXH |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.670 |
High resolution limit [Å] | 2.640 | 2.640 |
Number of reflections | 12559 | |
Completeness [%] | 91.9 | 67.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na/Cacodylate, MgCl2, PEG3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |