2E2L
Helicobacter pylori formamidase AmiF contains a fine-tuned cysteine-glutamate-lysine catalytic triad
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2006-03-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.089, 151.795, 89.083 |
| Unit cell angles | 90.00, 114.99, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.290 |
| R-factor | 0.25501 |
| Rwork | 0.253 |
| R-free | 0.28469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e2k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 77740 | |
| Completeness [%] | 91.4 | 92.5 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M lithium sulfate, 0.1M sodium acetate, 6 % PEG 1500, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
