2E26
Crystal structure of two repeat fragment of reelin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-11-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.008, 70.953, 94.769 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 47.290 - 2.000 |
| R-factor | 0.18125 |
| Rwork | 0.179 |
| R-free | 0.21884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ddu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.164 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.491 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.075 |
| Number of reflections | 54041 | |
| <I/σ(I)> | 9.6 | 3.5 |
| Completeness [%] | 96.4 | 98.73 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 7% PEG 3350, 75mM ammonium acetate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
