2E1T
Crystal structure of Dendranthema morifolium DmAT complexed with malonyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.646, 122.922, 69.848 |
| Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
| Resolution | 35.490 - 2.100 |
| R-factor | 0.19793 |
| Rwork | 0.196 |
| R-free | 0.23363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e1v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.401 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.214 |
| Number of reflections | 49363 | |
| <I/σ(I)> | 19.7 | 3.8 |
| Completeness [%] | 95.8 | 74.1 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PEG 8000, 10% glycerol, 0.12M ammonium sulfate, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
