2E1M

Crystal Structure of L-Glutamate Oxidase from Streptomyces sp. X-119-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-11-22
Detector	RIGAKU RAXIS V
Spacegroup name	P 61 2 2
Unit cell lengths	123.875, 123.875, 168.762
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.270 - 2.800
R-factor	0.248
Rwork	0.248
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f8r
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.080	0.489
Number of reflections	24388
<I/σ(I)>	34.043	4.779
Completeness [%]	100.0	100
Redundancy	10	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	278	100mM CAPS-buffer, 1200mM NaH2PO4, 800mM K2HPO4, 50mM LiSO4, 5mM DTT, 20mM KPB, 10mg/ml GOX, pH 10.50, VAPOR DIFFUSION, SITTING DROP, temperature 278K