2E0T

Crystal structure of catalytic domain of dual specificity phosphatase 26, MS0830 from Homo sapiens

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-03
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.976, 0.9794, 0.9642
Spacegroup name	C 1 2 1
Unit cell lengths	80.721, 40.176, 49.944
Unit cell angles	90.00, 110.35, 90.00

Refinement procedure

Resolution	37.840 - 1.670
R-factor	0.172
Rwork	0.172
R-free	0.21200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.670	1.670
Number of reflections	17050
<I/σ(I)>	22.2	7.45
Completeness [%]	96.9	77.7
Redundancy	3.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	0.1M HEPES-Na (pH 7.5), 0.8M Potassium sodium tartrate tetrahydrate, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	0.1M HEPES-Na (pH 7.5), 0.8M Potassium sodium tartrate tetrahydrate, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	0.1M HEPES-Na (pH 7.5), 0.8M Potassium sodium tartrate tetrahydrate, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K