2E0C
crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain7 at 2.0 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.880, 87.720, 75.720 |
Unit cell angles | 90.00, 91.37, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
R-factor | 0.2473 |
Rwork | 0.221 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dht |
RMSD bond length | 0.006 |
RMSD bond angle | 1.264 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.062 | 0.413 |
Number of reflections | 65823 | |
<I/σ(I)> | 11.7 | 2.7 |
Completeness [%] | 99.5 | 99.8 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | 14% PEG 10000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |