2DZV
Structure of mutant tryptophan synthase alpha-subunit (D146A) from a hyperthermophile, Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-16 |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.378, 76.500, 166.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.29000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1geq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.710 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.251 |
| Number of reflections | 21211 | |
| <I/σ(I)> | 11.5 | 3.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 0.1M MES-NAOH, 10-15% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
