2DYQ
Crystal Structure of the C-terminal Phophotyrosine Interaction Domain of Human APBB3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-19 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 82.292, 82.292, 104.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.256 |
| Rwork | 0.254 |
| R-free | 0.29800 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.250 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 4171 | |
| <I/σ(I)> | 51.3 | 8.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 18.4 | 20.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 25% PEG 3350, 0.2M Ammonium Acetate, 0.1M Tris-HCl, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
