2DY9

Crystal structure of nucleoside diphosphate kinase in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-12-26
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	69.835, 69.835, 106.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.920 - 2.010
Rwork	0.122
R-free	0.16800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cwk
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.920	2.080
High resolution limit [Å]	2.010	2.010
Number of reflections	19689
<I/σ(I)>	32.1
Completeness [%]	100.0	100
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Na Citrate, Bicine, 1mM ADP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K