2DY9
Crystal structure of nucleoside diphosphate kinase in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-12-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 63 |
Unit cell lengths | 69.835, 69.835, 106.897 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.920 - 2.010 |
Rwork | 0.122 |
R-free | 0.16800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cwk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.920 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 19689 | |
<I/σ(I)> | 32.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Na Citrate, Bicine, 1mM ADP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |