2DY2
Nitrite reductase pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.115 |
| Spacegroup name | H 3 |
| Unit cell lengths | 74.996, 74.996, 153.611 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.770 - 2.260 |
| Rwork | 0.192 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zv2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.583 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.770 | 51.230 | 2.380 |
| High resolution limit [Å] | 2.260 | 7.150 | 2.260 |
| Rmerge | 0.065 | 0.033 | 0.294 |
| Total number of observations | 2300 | 12157 | |
| Number of reflections | 15045 | ||
| <I/σ(I)> | 7.9 | 20.4 | 2.6 |
| Completeness [%] | 99.8 | 92.1 | 100 |
| Redundancy | 5.6 | 5.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Tris, MgCl2, PEG 1500, pH 6.0, vapor diffusion, hanging drop, temperature 298K |
