2DXL
Glycerophosphodiesterase from Enterobacter aerogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 164.462, 164.462, 164.462 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.15984 |
| Rwork | 0.158 |
| R-free | 0.19384 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.356 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 29959 | |
| <I/σ(I)> | 34.3 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.9M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
