2DXE

Crystal structure of nucleoside diphosphate kinase in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-03-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	69.802, 69.802, 107.093
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.080 - 1.700
R-factor	0.168
Rwork	0.168
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cwk
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.100	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	32493
<I/σ(I)>	39
Completeness [%]	99.7	99.3
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Na Citrate, Bicine, 1mM GDP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K