2DX1
Crystal structure of RhoGEF protein Asef
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9789, 0.9794, 0.9640 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.932, 79.818, 68.004 |
| Unit cell angles | 90.00, 123.25, 90.00 |
Refinement procedure
| Resolution | 34.610 - 2.360 |
| Rwork | 0.232 |
| R-free | 0.29900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 18703 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG3350, 0.2M MgCl2, 0.1M HEPES-HCl buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
