2DWZ

Structure of the Oncoprotein Gankyrin in Complex with S6 ATPase of the 26S Proteasome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	120
Detector technology	CCD
Collection date	2005-01-25
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	41.991, 92.319, 167.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.194
Rwork	0.194
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.073	0.313
Number of reflections	24356
Completeness [%]	92.6	85.3
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				VAPOR DIFFUSION