2DWR

Crystal structure of the human Wa rotavirus VP8* carbohydrate-recognising domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-16
Detector	MAR CCD 165 mm
Wavelength(s)	0.9794
Spacegroup name	P 32 2 1
Unit cell lengths	74.762, 74.762, 70.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.550 - 2.500
R-factor	0.17973
Rwork	0.177
R-free	0.23508
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model based on the structure of porcine CRW-8 VP8* that we have solved.
RMSD bond length	0.008
RMSD bond angle	1.106
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	64.600
High resolution limit [Å]	2.500
Number of reflections	8135

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	25% PEG 4000, 20% 2-propanol, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K