2DWQ
Thermus thermophilus YchF GTP-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-24 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.940, 54.357, 92.585 |
| Unit cell angles | 80.11, 76.46, 73.12 |
Refinement procedure
| Resolution | 37.040 - 2.950 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dby |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 18991 | |
| <I/σ(I)> | 6.0465 | 2.41909 |
| Completeness [%] | 95.5 | 83.6 |
| Redundancy | 1.83013 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 300 | 0.01M Cobaltous Chloride, 0.1M Sodium Acetate, 1M 1,6 Hexanediol, 4% Acetone, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
