2DWN
Crystal structure of the PriA protein complexed with oligonucleotides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-05 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 112.250, 112.250, 260.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.350 |
| Rwork | 0.281 |
| R-free | 0.33320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d7e |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.847 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 9392 | |
| <I/σ(I)> | 15.4 | 2.73 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 293 | 0.1M sodium citrate, 0.4M ammonium sulfate, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium citrate | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 2 | sodium citrate | ||
| 4 | 1 | 2 | ammonium sulfate |
