2DWM

Crystal structure of the PriA protein complexed with oligonucleotides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-16
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	112.660, 112.660, 260.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.150
Rwork	0.271
R-free	0.31770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d7e
RMSD bond length	0.009
RMSD bond angle	24.463
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.150	3.150
Number of reflections	11411
<I/σ(I)>	15.5	4.77
Completeness [%]	99.9	100
Redundancy	10.8	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	293	0.1M sodium citrate, 0.2M ammonium sulfate, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	sodium citrate
2	1	1	ammonium sulfate
3	1	2	sodium citrate
4	1	2	ammonium sulfate