2DWI
Crystal structure of the complex formed between C-terminal half of bovine lactoferrin and cellobiose at 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.472, 50.439, 65.930 |
| Unit cell angles | 90.00, 107.78, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.18566 |
| Rwork | 0.184 |
| R-free | 0.22068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.441 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 20254 | |
| <I/σ(I)> | 6.8 | 2.1 |
| Completeness [%] | 99.5 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M MES, 25% POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 0.01M ZINC SULPHATE, pH 6.5, VAPOR DIFFUSION, temperature 298K |
