2DWG

RUN domain of Rap2 interacting protein x, crystallized in P2(1)2(1)2(1) space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-10-01
Detector	RIGAKU
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.625, 77.674, 88.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.020 - 3.220
R-factor	0.21
Rwork	0.210
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1WUS
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	6828
<I/σ(I)>	11.6818	3.92199
Completeness [%]	99.5	97.7
Redundancy	6.49238

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	300	1.4M lithium sulfate, 0.1M HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K