2DVL
Crystal structure of project TT0160 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-14 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 116.479, 116.479, 149.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1JQI |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | d*TREK |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.083 | 0.356 |
| Number of reflections | 72738 | |
| <I/σ(I)> | 17 | 7.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 13.97 | 14.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 0.1M Ammonium dihydrogen phosphate, 0.1M Tris HCl, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
