2DVE
Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii OT3 in Complex with Biotinyl-5'-AMP, Mutation Arg51Ala
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.349, 82.744, 72.596 |
| Unit cell angles | 90.00, 102.43, 90.00 |
Refinement procedure
| Resolution | 37.450 - 1.600 |
| R-factor | 0.1926 |
| Rwork | 0.193 |
| R-free | 0.20840 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1wqw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.119 |
| Number of reflections | 54116 | |
| <I/σ(I)> | 25.3 | 5.72 |
| Completeness [%] | 92.9 | 61.4 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.2 | 295 | PEG20K, Acetate, NaOH, ATP, biotin, pH 5.2, microbatch, temperature 295K |
