2DUY
Crystal structure of competence protein ComEA-related protein from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9724 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.635, 42.635, 53.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.750 |
R-factor | 0.193 |
Rwork | 0.188 |
R-free | 0.21800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.062 | 0.310 |
Number of reflections | 5420 | |
<I/σ(I)> | 69.2 | 10.2 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 26 | 19.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 1.5M Ammonium citrate, 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 1.5M Ammonium citrate, 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 1.5M Ammonium citrate, 0.1M Bis-tris propane, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |