2DUU
Crystal Structure of apo-form of NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase from Synechococcus Sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.400, 79.800, 207.200 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.217 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d2i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.075 | 0.340 |
| Number of reflections | 50999 | |
| <I/σ(I)> | 4.5 | 2 |
| Completeness [%] | 94.4 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | gel-tube method | 4.8 | 293 | 60% saturation of ammonium sulfate, 0.1M citrate buffer, 0.2M potassium sodium tartrate, pH 4.8, gel-tube method, temperature 293K |
