2DUT

Crystal structure of a M-loop deletion variant of MENT in the native conformation

Replaces:  2H4S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-04
Detector	ADSC QUANTUM 4
Spacegroup name	P 32
Unit cell lengths	182.559, 182.559, 96.723
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.278
Rwork	0.278
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ova
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.114	0.512
Number of reflections	68817
<I/σ(I)>	10.8	2.8
Completeness [%]	95.8	99.9
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	290	1.7-1.9M AMMONIUM SULFATE, 0.1M SODIUM ACETATE, PH4.3-4.45, VAPOR DIFFUSION, HANGING DROP, temperature 290K