2DUR
Crystal structure of VIP36 exoplasmic/lumenal domain, Ca2+/Man2-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.200, 45.500, 117.400 |
| Unit cell angles | 90.00, 132.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2duq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.318 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.051 | 0.286 |
| Number of reflections | 79197 | |
| <I/σ(I)> | 14.4 | 3.4 |
| Completeness [%] | 98.4 | 91.1 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 5% PEG4000, 0.3M MgCl2, 3.4mM mannobiose, 0.1M MES (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K |
