2DUJ
Crystal structure of the complex formed between proteinase K and a synthetic peptide Leu-Leu-Phe-Asn-Asp at 1.67 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.249, 68.249, 108.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 1.670 |
| R-factor | 0.16425 |
| Rwork | 0.163 |
| R-free | 0.19235 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.726 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 30360 | |
| Completeness [%] | 99.8 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | sodium nitrate, CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
