2DU7
Crystal structure of Methanococcus jannacshii O-phosphoseryl-tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2005-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 196.354, 299.455, 125.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 3.600 |
| R-factor | 0.33 |
| Rwork | 0.330 |
| R-free | 0.38700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2du3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 42030 | |
| Completeness [%] | 97.6 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 11.25% PEG 4000, 75mM Na-citrate, 75mM ADA-NaOH buffer (pH 6.7), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
