2DU1
Crystal Structure of basic winged bean lectin in complex with Methyl-alpha-N-acetyl-D galactosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 157.619, 90.960, 73.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.25100 |
| Starting model (for MR) | 1wbl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.135 | 0.438 |
| Number of reflections | 31848 | |
| <I/σ(I)> | 24.3 | 5.8 |
| Completeness [%] | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 300 | 20% PEG 4000, 10% Iso-propenol, 0.02M PBS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
